Journal:

Article Title: Sensitive and Specific Method for Rapid Identification of Streptococcus pneumoniae Using Real-Time Fluorescence PCR

doi: 10.1128/JCM.39.10.3446-3451.2001

Figure Lengend Snippet: Serial dilutions of S. pneumoniae (ATCC 33400) DNA isolated by a standardized method and quantitated spectrophotometrically to 4.4e6 through 4.4e0 genomic equivalents were used for determination of real-time PCR assay detection limits or in vitro sensitivity testing. Cycle number plotted against the log of calculated concentration values resulted in a standard curve with an error of 0.592 and correlation coefficient at unity. Human genomic DNA at 4,500 genomic equivalents and NTC samples did not fluoresce above background signal. The detection limits of the PCR assay demonstrated similar results when the dilution series panel was run in testing of all cross-reaction panel and unknown organisms.

Article Snippet: The detection limits of the PCR assay demonstrated similar results when the dilution series panel was run in testing of all cross-reaction panel and unknown organisms. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Genus Species or serovar(s) (subtypes) Homo H. sapiens Streptococcus S. agalactiae, S. bovis, S. equi, S. equisimilis, S. pyogenes, S. sanguis (type II) Campylobacter C. coli, C. lari, C. jejuni Citrobacter C. freundii Escherichia E. coli H1, O28, O55, O111, 112, 0126, 0128, O157:H7 Klebsiella K. pneumoniae Leclercia L. adecarboxylata Neisseria N. lactamica Proteus P. vulgaris Pseudomonas P. aeruginosa Salmonella Bovis-morbificus, Choleraesuis, Cubana, enteritidis, Heidelberg, Infantis, Javiana, Lanka, Kovka, Montevideo, Newport, Paratyphi-A, Poona Typhi-1 (1078), Typhimurium (528) Shigella S. flexneri, S. boydii (type I) S. dysenteriae (type III) Staphylococcus S. aureus Mycoplasma M. pneumoniae, M. hominis Open in a separate window Cross-reactivity panel: negative-control organisms In addition, 1.0 ng of genomic DNA from laboratory stock, as well as DNA purified by the modified capture disk method, from each of 10 S. pneumoniae strains (ATCC 6305, ATCC 49619, ATCC 33400, ATCC 51915, 1301, 1346, 1518, 1661, 1830, and 2113) were correctly identified by the PCR assay.

Techniques: Isolation, Real-time Polymerase Chain Reaction, In Vitro, Concentration Assay

Journal:

Article Title: Sensitive and Specific Method for Rapid Identification of Streptococcus pneumoniae Using Real-Time Fluorescence PCR

doi: 10.1128/JCM.39.10.3446-3451.2001

Figure Lengend Snippet: Cross-reactivity panel: negative-control organisms

Article Snippet: The detection limits of the PCR assay demonstrated similar results when the dilution series panel was run in testing of all cross-reaction panel and unknown organisms. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Genus Species or serovar(s) (subtypes) Homo H. sapiens Streptococcus S. agalactiae, S. bovis, S. equi, S. equisimilis, S. pyogenes, S. sanguis (type II) Campylobacter C. coli, C. lari, C. jejuni Citrobacter C. freundii Escherichia E. coli H1, O28, O55, O111, 112, 0126, 0128, O157:H7 Klebsiella K. pneumoniae Leclercia L. adecarboxylata Neisseria N. lactamica Proteus P. vulgaris Pseudomonas P. aeruginosa Salmonella Bovis-morbificus, Choleraesuis, Cubana, enteritidis, Heidelberg, Infantis, Javiana, Lanka, Kovka, Montevideo, Newport, Paratyphi-A, Poona Typhi-1 (1078), Typhimurium (528) Shigella S. flexneri, S. boydii (type I) S. dysenteriae (type III) Staphylococcus S. aureus Mycoplasma M. pneumoniae, M. hominis Open in a separate window Cross-reactivity panel: negative-control organisms In addition, 1.0 ng of genomic DNA from laboratory stock, as well as DNA purified by the modified capture disk method, from each of 10 S. pneumoniae strains (ATCC 6305, ATCC 49619, ATCC 33400, ATCC 51915, 1301, 1346, 1518, 1661, 1830, and 2113) were correctly identified by the PCR assay.

Techniques: Negative Control

Journal:

Article Title: Sensitive and Specific Method for Rapid Identification of Streptococcus pneumoniae Using Real-Time Fluorescence PCR

doi: 10.1128/JCM.39.10.3446-3451.2001

Figure Lengend Snippet: Results of double blind PCR-based testing of clinical isolates

Article Snippet: The detection limits of the PCR assay demonstrated similar results when the dilution series panel was run in testing of all cross-reaction panel and unknown organisms. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Genus Species or serovar(s) (subtypes) Homo H. sapiens Streptococcus S. agalactiae, S. bovis, S. equi, S. equisimilis, S. pyogenes, S. sanguis (type II) Campylobacter C. coli, C. lari, C. jejuni Citrobacter C. freundii Escherichia E. coli H1, O28, O55, O111, 112, 0126, 0128, O157:H7 Klebsiella K. pneumoniae Leclercia L. adecarboxylata Neisseria N. lactamica Proteus P. vulgaris Pseudomonas P. aeruginosa Salmonella Bovis-morbificus, Choleraesuis, Cubana, enteritidis, Heidelberg, Infantis, Javiana, Lanka, Kovka, Montevideo, Newport, Paratyphi-A, Poona Typhi-1 (1078), Typhimurium (528) Shigella S. flexneri, S. boydii (type I) S. dysenteriae (type III) Staphylococcus S. aureus Mycoplasma M. pneumoniae, M. hominis Open in a separate window Cross-reactivity panel: negative-control organisms In addition, 1.0 ng of genomic DNA from laboratory stock, as well as DNA purified by the modified capture disk method, from each of 10 S. pneumoniae strains (ATCC 6305, ATCC 49619, ATCC 33400, ATCC 51915, 1301, 1346, 1518, 1661, 1830, and 2113) were correctly identified by the PCR assay.

Techniques: Negative Control

Journal: Journal of Clinical Microbiology

Article Title: Performance assessment of the Bruker Biotyper MALDI-TOF MS for the identification of difficult-to-identify viridans group streptococci

doi: 10.1128/jcm.01143-23

Figure Lengend Snippet: Identification results of the three Bruker Biotyper systems for the reference strains

Article Snippet: TABLE 1 Group Strain 16S rDNA (%) a MALDI TOF MS Biotyper 3.1 (DB_5627) Biotyper 3.1 (DB_6903) Biotyper 4.1 (DB_10833) ID b Group ID b Group ID b Group Mitis S. mitis ATCC 49456 100 Spn Mitis Smi Mitis Smi Mitis Mitis S. oralis ATCC 35037 100 Spn Mitis Sor Mitis Sor Mitis Mitis S. pseudopneumoniae ATCC BAA-960 100 Spn Mitis Sps Mitis Sps Mitis Mitis S. pneumoniae ATCC 49619 100 ND c ND c Spn Mitis Spn Mitis Mitis S. pneumoniae ATCC 6301 100 ND c ND c Spn Mitis Spn Mitis Mitis S. pneumoniae ATCC 6305 100 ND c ND c Spn Mitis Spn Mitis Mitis S. pneumoniae ATCC BAA-255 100 ND c ND c Spn Mitis Spn Mitis Mitis S. pneumoniae ATCC 33400 100 ND c ND c Spn Mitis Spn Mitis Bovis S. gallolyticus ATCC 43144 100 ND c ND c Sga Bovis Sga Bovis Bovis S. equinus ATCC 33317 100 ND c ND c Slu Bovis Slu Bovis Bovis S. infantarius ATCC BAA-102 100 ND c ND c Seq Bovis Sinf Bovis Bovis S. lutetiensis ATCC BAA-103 99.93 ND c ND c Slu Bovis Slu Bovis Anginosus S. anginosus ATCC 9895 100 San Anginosus San Anginosus San Anginosus Anginosus S. constellatus ATCC 27513 100 Sco Anginosus Sco Anginosus Sco Anginosus Anginosus S. intermedius ATCC 27335 100 Sint Anginosus Sint Anginosus Sint Anginosus Sanguinis S. sanguinis ATCC 10556 100 Ssan Sanguinis Ssan Sanguinis Ssan Sanguinis Sanguinis S. parasanguinis ATCC 15909 99.86 Spa Sanguinis Spa Sanguinis Spa Sanguinis Sanguinis S. gordonii ATCC 35105 100 Sgo Sanguinis Sgo Sanguinis Sgo Sanguinis Salivarius S. salivarius ATCC 7073 100 Ssal Salivarius Ssal Salivarius Ssal Salivarius Mutans S. mutans ATCC GS-5 100 Smu Mutans Smu Mutans Smu Mutans Open in a separate window a Percent homology to 16S rDNA sequences in the NCBI database. b The text in bold indicates discrepant results.

Techniques:

Journal: Journal of Clinical Microbiology

Article Title: Performance assessment of the Bruker Biotyper MALDI-TOF MS for the identification of difficult-to-identify viridans group streptococci

doi: 10.1128/jcm.01143-23

Figure Lengend Snippet: Accuracy ratios of VGS species identification among clinical isolates using three Bruker Biotyper systems

Article Snippet: TABLE 1 Group Strain 16S rDNA (%) a MALDI TOF MS Biotyper 3.1 (DB_5627) Biotyper 3.1 (DB_6903) Biotyper 4.1 (DB_10833) ID b Group ID b Group ID b Group Mitis S. mitis ATCC 49456 100 Spn Mitis Smi Mitis Smi Mitis Mitis S. oralis ATCC 35037 100 Spn Mitis Sor Mitis Sor Mitis Mitis S. pseudopneumoniae ATCC BAA-960 100 Spn Mitis Sps Mitis Sps Mitis Mitis S. pneumoniae ATCC 49619 100 ND c ND c Spn Mitis Spn Mitis Mitis S. pneumoniae ATCC 6301 100 ND c ND c Spn Mitis Spn Mitis Mitis S. pneumoniae ATCC 6305 100 ND c ND c Spn Mitis Spn Mitis Mitis S. pneumoniae ATCC BAA-255 100 ND c ND c Spn Mitis Spn Mitis Mitis S. pneumoniae ATCC 33400 100 ND c ND c Spn Mitis Spn Mitis Bovis S. gallolyticus ATCC 43144 100 ND c ND c Sga Bovis Sga Bovis Bovis S. equinus ATCC 33317 100 ND c ND c Slu Bovis Slu Bovis Bovis S. infantarius ATCC BAA-102 100 ND c ND c Seq Bovis Sinf Bovis Bovis S. lutetiensis ATCC BAA-103 99.93 ND c ND c Slu Bovis Slu Bovis Anginosus S. anginosus ATCC 9895 100 San Anginosus San Anginosus San Anginosus Anginosus S. constellatus ATCC 27513 100 Sco Anginosus Sco Anginosus Sco Anginosus Anginosus S. intermedius ATCC 27335 100 Sint Anginosus Sint Anginosus Sint Anginosus Sanguinis S. sanguinis ATCC 10556 100 Ssan Sanguinis Ssan Sanguinis Ssan Sanguinis Sanguinis S. parasanguinis ATCC 15909 99.86 Spa Sanguinis Spa Sanguinis Spa Sanguinis Sanguinis S. gordonii ATCC 35105 100 Sgo Sanguinis Sgo Sanguinis Sgo Sanguinis Salivarius S. salivarius ATCC 7073 100 Ssal Salivarius Ssal Salivarius Ssal Salivarius Mutans S. mutans ATCC GS-5 100 Smu Mutans Smu Mutans Smu Mutans Open in a separate window a Percent homology to 16S rDNA sequences in the NCBI database. b The text in bold indicates discrepant results.

Techniques:

Journal: Journal of Clinical Microbiology

Article Title: Comparison of Conventional, Nested, and Real-Time Quantitative PCR for Diagnosis of Scrub Typhus ▿

doi: 10.1128/JCM.01216-09

Figure Lengend Snippet: Bacterial strains used in this study

Article Snippet: The rickettsial strains were obtained from the Australian Rickettsial Reference Laboratory (ARRL). table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Strain Pathogen 1 Aeromonas caviae ATCC 15468 2 Aeromonas hydrophila subsp. hydrophila ATCC 7966 3 Aeromonas hydrophila subsp. anaerogenes ATCC 15467 4 Vibrio alginolyticus ATCC 17749 5 Vibrio cholerae ATCC14035 6 Vibrio fluvialis ATCC 33809 7 Vibrio furnissii ATCC 35016 8 Vibrio hollisae ATCC 33564 9 Vibrio mimicus ATCC 33653 10 Vibrio parahaemolyticus ATCC 17802 11 Vibrio proteolyticus ATCC 15338 12 Vibrio vulnificus ATCC 27562 13 Streptococcus agalactiae ATCC 13813 14 Streptococcus mitis ATCC 49456 15 Streptococcus mutans ATCC 15175 16 Streptococcus pneumoniae ATCC 33400 17 Streptococcus pyogenes ATCC 12344 18 Streptococcus salivarius ATCC 7073 19 Streptococcus sanguinis ATCC 10556 20 Streptococcus sobrinus ATCC 6715 21 Staphylococcus aureus subsp. aureus (MRSA a ) ATCC 33591 22 Staphylococcus aureus (MRSA) ATCC 29213 23 Staphylococcus epidermidis ATCC 12228 24 Staphylococcus saprophyticus subsp. saprophyticus ATCC 15305 25 Salmonella enterica serovar Typhimurium KCTC 1925 26 Klebsiella pneumoniae ATCC 13883 27 Shigella sonnei ATCC 25931 28 Pseudomonas aeruginosa ATCC 27853 29 Clostridium perfringens ATCC 3624 30 Aeromonas salmonicida subsp. salmonicida ATCC 33658 31 Clostridium difficile ATCC 9689 32 Rickettsia honei RB 33 Rickettsia rickettsii Smith 34 Rickettsia conorii 7 35 Rickettsia akari MK (Kaplan) 36 Rickettsia prowazekii Breinl 37 Rickettsia sibirica 246 38 Rickettsia australis JC 39 Rickettsia typhi Wilmington 40 Leptospira interrogans 41 Orientia tsutsugamushi Kato 42 Orientia tsutsugamushi Karp 43 Orientia tsutsugamushi Gilliam Open in a separate window a MRSA, methicillin-resistant S. aureus .

Techniques: